• Users Online: 1433
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2017  |  Volume : 1  |  Issue : 1  |  Page : 13-15

Impregnation and embedding using bees wax and paraffin wax in oral tissue samples: A comparative study


Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu, India

Correspondence Address:
J Vini Mary Antony
Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijofb.ijofb_3_17

Rights and Permissions

Aim: The aim of this study is to compare paraffin wax that is used as routine embedding media and beeswax in impregnation and embedding of oral tissues. Materials and Methods: Ten biopsy specimens were impregnated and embedded in paraffin wax, ten biopsy specimens were impregnated and embedded in beeswax. After manual processing, all sections were stained with Hematoxylin and Eosin to compare the effect of beeswax and paraffin wax based on the features of the integrity of the section, uniformity of the stain, staining which includes nuclear details, cytoplasmic details, and background staining. Results: Beeswax showed well impregnation and embedding of the tissues as well as the preservation of the nuclear details, good cytoplasmic appearance, good tissue architecture and no bad effect on staining characteristics of the tissue. In addition, beeswax reduced the time needed for wax cooling. Conclusion: Beeswax can be used as an alternative to paraffin wax. Thus, we recommended using beeswax in our laboratories as they are widely available.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed40195    
    Printed662    
    Emailed0    
    PDF Downloaded925    
    Comments [Add]    

Recommend this journal