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Year : 2020  |  Volume : 4  |  Issue : 1  |  Page : 10-14

Stains employed in the detection of microorganisms

1 Department of Oral Pathology, SRM Dental College, Chennai, Tamil Nadu, India
2 Private Practitioner, Chennai, Tamil Nadu, India

Correspondence Address:
E Abigail Viola
Department of Oral Pathology, SRM Dental College, Chennai, Tamil Nadu
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ijofb.ijofb_5_22

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A clear diagnosis of infection can often be made based on the macroscopic appearance of tissue. Infection can be detected by frank pus, abscess formation, cavitation, hyperkeratosis, demyelination, pseudo-membrane formation, focal necrosis, and granulomas. Even if the precise nature of the suspect organism is never identified, some of these appearances may be sufficient to provide an initial, or at least provisional, diagnosis and allow treatment to begin, even if the precise nature of the suspect organism is never identified. It is important to remember that a well-executed hematoxylin and eosin method will stain a wide range of organisms. Romanowsky stains, such as Giemsa and Papanicolaou stains will also stain organisms and their cellular environment. Other infectious agents are difficult to detect using standard stains, necessitating the use of special techniques to demonstrate their presence. This review aims to highlight the commonly used stains for the detection of microorganisms in various histopathology and cytology material.

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